The overall objective of this course is to provide a solid training on the most popular genome editing techniques used in genetic engineering and plant biotechnology.
Plant biotechnology has promptly developed into one of the most prolific, expanding and influential areas of the plant sciences. Applications of modern biotechnological tools have resulted in great advances for agriculture and society. The overall objective of this course is to provide basic training on the use of biotechnological tools for plant improvement. Principles, state of the art and applications of tools to improve plant sustainability, performance and tolerance to biotic and abiotic stresses are covered.
This course provides students with hands-on experience in the most basic laboratory methods used to characterize nucleic acid sequences and proteins. Instruction is based on a balanced combination of lectures, direct experimentation, and preparation of lab reports. Students will learn to measure plant genome sizes and use this information for a variety of applications in molecular biology and genomics. Students will also learn to: use current bioinformatics resources to identify specific DNA sequences, design primers for PCR amplification, and clone and sequence amplification products. Labs are designed for the isolation and characterization of DNA, RNA and proteins using standard technologies. In addition, the class will carry out transient and stable plant transformation experiments.