University of Florida

Seed Bank Contribution to Natural Recolonization

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Two Ponds sampled for seed banks
Two ponds in the Florida Panther National Wildlife Refuge, Naples, Florida, were sampled to characterize spatial and depth heterogeneity of their seed banks.
pond s divided into concentric zones
Ponds were divided into three concentric zones based on vegetation type and eight radial study plots.
3 soil samples taken in each zone
Three soil samples were collected in each zone along each study plot for the spatial recolonization experiment.
Pond soil sample depth profiles
Three soil sample depth profiles were collected in each zone along one study plot for each pond for the depth recolonization experiment.
rhizomes and roots removed from soil samples
All material with the potential to vegetatively propagate was removed from each soil sample (rhizomes and roots).
trays filled with rockwool and potting soil
Tray were filled with 2-cm of absorbant Rockwool and 2-cm of potting soil.
soil spread over potting soil and rockwool
The soil was spread 1-cm thick on top of 2-cm of potting soil and 2-cm of Rockwool.
trays immersed in water
Trays were immersed in 4-5-cm of water to maintain ideal moisture levels for germination.
plants kept in trays
Plants will be kept in trays until they become identifiable, and then will be removed and planted in a separate bench to continue growing.